Impact of long-term storage and freeze-thawing on eight circulating microRNAs in plasma samples.

ABSTRACT: Sample collection, processing, storage and isolation methods constitute pre-analytic factors that can influence the quality of samples used in research and clinical practice. With regard to biobanking practices, a critical point in the sample's life chain is storage, particularly long-term storage. Since most studies examine the influence of different temperatures (4°C, room temperature) or delays in sample processing on sample quality, there is only little information on the effects of long-term storage at ultra-low (vapor phase of liquid nitrogen) temperatures on biomarker levels. Among these biomarkers, circulating miRNAs hold great potential for diagnosis or prognosis for a variety of diseases, like cancer, infections and chronic diseases, and are thus of high interest in several scien

SUBMITTER: Matias-Garcia PR 

PROVIDER: S-EPMC6959605 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature