Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m⁶A modification.

ABSTRACT: Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a mutant defective in mRNA methylation (m⁶A). Here we show that m⁶A can be mapped in full-length mRNAs transcriptome-wide and reveal the combinatorial diversity of cap-associated transcription start sites, splicing events, poly(A) site choice and poly(A) tail length. Loss of m⁶A from 3' untranslated regions is associated with decreased relative transcript abundance and defective RNA 3' end formation. A functional consequence of disrupted m⁶A is a lengthening of the circadian period. We conclu

SUBMITTER: Parker MT 

PROVIDER: S-EPMC6959997 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature