Illumination of PRRSV Cytotoxic T Lymphocyte Epitopes by the Three-Dimensional Structure and Peptidome of Swine Lymphocyte Antigen Class I (SLA-I).

ABSTRACT: To investigate CTL epitope applications in swine, SLA-1^*1502-restricted peptide epitopes matching porcine reproductive and respiratory syndrome virus (PRRSV) strains were explored by crystallography, biochemistry, and the specific pathogen-free (SPF) swine experiments. First, nine predicted PRRSV peptides were tested by assembly of the peptide-SLA-1^*1502 (pSLA-1^*1502) complexes, and the crystal structure of the SLA-1^*1502 complex with one peptide (NSP9-TMP9) was determined. The NSP9-TMP9 peptide conformation presented by pSLA-1^*1502 is different from that of the peptides presented by the known pSLA-1^*0401 and pSLA-3^*hs0202 complexes. Two consecutive Pro residues make the turn between P3 and P4 of NSP9-TMP9 much sh

SUBMITTER: Pan X 

PROVIDER: S-EPMC6960135 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature