Development of protocols for the first serial block-face scanning electron microscopy (SBF SEM) studies of bone tissue.

ABSTRACT: There is an unmet need for a high-resolution three-dimensional (3D) technique to simultaneously image osteocytes and the matrix in which these cells reside. In serial block-face scanning electron microscopy (SBF SEM), an ultramicrotome mounted within the vacuum chamber of a microscope repeatedly sections a resin-embedded block of tissue. Backscattered electron scans of the block face provide a stack of high-resolution two-dimensional images, which can be used to visualise and quantify cells and organelles in 3D. High-resolution 3D images of biological tissues from SBF SEM have been exploited considerably to date in the neuroscience field. However, non-brain samples, in particular hard biological tissues, have appeared more challenging to image by SBF SEM due to the difficulties of sectioni

SUBMITTER: Goggin P 

PROVIDER: S-EPMC6961117 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature