LncRNA JPX/miR-33a-5p/Twist1 axis regulates tumorigenesis and metastasis of lung cancer by activating Wnt/β-catenin signaling.

ABSTRACT:

Background

MicroRNAs (miRNAs) and Twist1-induced epithelial-mesenchymal transition (EMT) in cancer cell dissemination are well established, but the involvement of long noncoding RNAs (lncRNAs) in Twist1-mediated signaling remains largely unknown.

Methods

RT-qPCR and western blotting were conducted to detect the expression levels of lncRNA JPX and Twist1 in lung cancer cell lines and tissues. The impact of JPX on Twist1 expression, cell growth, invasion, apoptosis, and in vivo tumor growth were investigated in lung cancer cells by western blotting, rescue experiments, colony formation assay, flow cytometry, and xenograft animal experiment.

Results

We observed that lncRNA JPX was upregulated in lung cancer metastatic tissues and was closely correlated with tumor size an

SUBMITTER: Pan J 

PROVIDER: S-EPMC6961326 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature