The L-platform/L-scaffold framework: a blueprint for RNA-cleaving nucleic acid enzyme design.

ABSTRACT: We develop an L-platform/L-scaffold framework we hypothesize may serve as a blueprint to facilitate site-specific RNA-cleaving nucleic acid enzyme design. Building on the L-platform motif originally described by Suslov and coworkers, we identify new critical scaffolding elements required to anchor a conserved general base guanine ("L-anchor") and bind functionally important metal ions at the active site ("L-pocket"). Molecular simulations, together with a broad range of experimental structural and functional data, connect the L-platform/L-scaffold elements to necessary and sufficient conditions for catalytic activity. We demonstrate that the L-platform/L-scaffold framework is common to five of the nine currently known naturally occurring ribozyme classes (Twr, HPr, VSr, HHr, Psr), and intr

SUBMITTER: Gaines CS 

PROVIDER: S-EPMC6961537 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature