A genome-wide long noncoding RNA CRISPRi screen identifies <i>PRANCR</i> as a novel regulator of epidermal homeostasis.

ABSTRACT: Genome-wide association studies indicate that many disease susceptibility regions reside in non-protein-coding regions of the genome. Long noncoding RNAs (lncRNAs) are a major component of the noncoding genome, but their biological impacts are not fully understood. Here, we performed a CRISPR interference (CRISPRi) screen on 2263 epidermis-expressed lncRNAs and identified nine novel candidate lncRNAs regulating keratinocyte proliferation. We further characterized a top hit from the screen, progenitor renewal associated non-coding RNA (PRANCR), using RNA interference-mediated knockdown and phenotypic analysis in organotypic human tissue. PRANCR regulates keratinocyte proliferation, cell cycle progression, and clonogenicity. PRANCR-deficient epidermis displayed impaired

SUBMITTER: Cai P 

PROVIDER: S-EPMC6961571 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature