Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9.

ABSTRACT: Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and s

SUBMITTER: Sun Y 

PROVIDER: S-EPMC6961580 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature