Potentiation of curing by a broad-host-range self-transmissible vector for displacing resistance plasmids to tackle AMR.

ABSTRACT: Plasmids are potent vehicles for spread of antibiotic resistance genes in bacterial populations and often persist in the absence of selection due to efficient maintenance mechanisms. We previously constructed non-conjugative high copy number plasmid vectors that efficiently displace stable plasmids from enteric bacteria in a laboratory context by blocking their replication and neutralising their addiction systems. Here we assess a low copy number broad-host-range self-transmissible IncP-1 plasmid as a vector for such curing cassettes to displace IncF and IncK plasmids. The wild type plasmid carrying the curing cassette displaces target plasmids poorly but derivatives with deletions near the IncP-1 replication origin that elevate copy number about two-fold are efficient. Verification of thi

SUBMITTER: Lazdins A 

PROVIDER: S-EPMC6961859 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature