Stable isotope metabolomics of pulmonary artery smooth muscle and endothelial cells in pulmonary hypertension and with TGF-beta treatment.

ABSTRACT: Altered metabolism in pulmonary artery smooth muscle cells (PASMCs) and endothelial cells (PAECs) contributes to the pathology of pulmonary hypertension (PH), but changes in substrate uptake and how substrates are utilized have not been fully characterized. We hypothesized stable isotope metabolomics would identify increased glucose, glutamine and fatty acid uptake and utilization in human PASMCs and PAECs from PH versus control specimens, and that TGF-β treatment would phenocopy these metabolic changes. We used ¹³C-labeled glucose, glutamine or a long-chain fatty acid mixture added to cell culture media, and mass spectrometry-based metabolomics to detect and quantify ¹³C-labeled metabolites. We found PH PASMCs had increased glucose uptake and utilization by glycolysi

SUBMITTER: Hernandez-Saavedra D 

PROVIDER: S-EPMC6962446 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature