Clinical Targeted Next-Generation sequencing Panels for Detection of Somatic Variants in Gliomas.

ABSTRACT:

Purpose

Targeted next-generation sequencing (NGS) panels for solid tumors have been useful in clinical framework for accurate tumor diagnosis and identifying essential molecular aberrations. However, most cancer panels have been designed to address a wide spectrum of pan-cancer models, lacking integral prognostic markers that are highly specific to gliomas.

Materials and methods

To address such challenges, we have developed a glioma-specific NGS panel, termed "GliomaSCAN," that is capable of capturing single nucleotide variations and insertion/deletion, copy number variation, and selected promoter mutations and structural variations that cover a subset of intron regions in 232 essential glioma-associated genes. We confirmed clinical concordance rate using pairwise comparison

SUBMITTER: Shin H 

PROVIDER: S-EPMC6962483 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature