Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation.

ABSTRACT: We have previously established a concept of developing exogenic pancreas in a genetically modified pig fetus with an apancreatic trait, thereby proposing the possibility of in vivo generation of functional human organs in xenogenic large animals. In this study, we aimed to demonstrate a further proof-of-concept of the compensation for disabled organogeneses in pig, including pancreatogenesis, nephrogenesis, hepatogenesis, and vasculogenesis. These dysorganogenetic phenotypes could be efficiently induced via genome editing of the cloned pigs. Induced dysorganogenetic traits could also be compensated by allogenic blastocyst complementation, thereby proving the extended concept of organ regeneration from exogenous pluripotent cells in empty niches during various organogeneses. These results s

SUBMITTER: Matsunari H 

PROVIDER: S-EPMC6962638 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature