Characterization of human telomerase reverse transcriptase immortalized anterior cruciate ligament cell lines.

ABSTRACT: BACKGROUND:The anterior-cruciate-ligament (ACL) contains mesenchymal stem cells (ACL-MSCs), suggesting the feasibility of regenerative treatments of this tissue. The immortalization of isolated cells results in cell-lines applicable to develop cell-based therapies. Immortal cell lines eliminate the need for frequent cell isolation from donor tissues. The objective of this study was to characterize cell lines that were generated from isolated ACL-MSCs using TERT gene transfer. METHODS:We isolated ACL-MSCs from human ACLs derived at the time of ACL reconstruction surgery or total knee arthroplasty. We generated cell lines and compared them to non-immortalized ACL-MSCs. We assessed the cellular morphology and we detected surface antigen expression. The resistance to senescence was inferred us

SUBMITTER: Prager P 

PROVIDER: S-EPMC6962762 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature