Genome-wide SNP and InDel mutations in Mycobacterium tuberculosis associated with rifampicin and isoniazid resistance.

ABSTRACT:

Objective

Multiple resistances to isoniazid and rifampicin lead to the majority of death associated with M. tuberculosis infection. This study aimed to characterize the single nucleotide polymorphisms (SNPs) and insertion and deletion (InDel) mutations associated with isoniazid and rifampicin resistance.

Methods

The M. tuberculosis strain H37Rv was cultured and treated with isoniazid or rifampicin for generations. Total DNA samples from different generations were extracted for construction of DNA library, and the SNP and InDel mutation in different samples were detected by whole genome sequencing. Bioinformatics analysis such as phylogenetic tree and heap map were also performed.

Results

Totally 58 nonsynonymous SNP mutations, 64 synonymous SNP mutations

SUBMITTER: Li H 

PROVIDER: S-EPMC6962771 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature