Ovarian IL-1α and IL-1β levels are associated with primary ovarian insufficiency.

ABSTRACT: Primary ovarian insufficiency (POI) is characterized by premature ovarian failure and lack of menstrual periods in women under the age of 40. Ample evidence suggests that interleukin 1 (IL-1) family proteins are involved in the processes of ovarian follicular development and depletion. The association of these cytokines with POI disorder and the underlying molecular mechanism in regulation of the ovarian functions remain largely undetermined. In this study, follicular fluids and serum samples were harvested from POI patients and healthy women who underwent follicular aspiration during in vitro fertilization. Enzyme-linked immunosorbent assay (ELISA) and the quantitative real-time PCR (qPCR) were employed to assess the levels of protein secretion and mRNA expression of IL-1α and IL-1β, as w

SUBMITTER: Yang H 

PROVIDER: S-EPMC6962969 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature