NTRC and Thioredoxin f Overexpression Differentially Induces Starch Accumulation in Tobacco Leaves.

ABSTRACT: Thioredoxin (Trx) f and NADPH-dependent Trx reductase C (NTRC) have both been proposed as major redox regulators of starch metabolism in chloroplasts. However, little is known regarding the specific role of each protein in this complex mechanism. To shed light on this point, tobacco plants that were genetically engineered to overexpress the NTRC protein from the chloroplast genome were obtained and compared to previously generated Trx f-overexpressing transplastomic plants. Likewise, we investigated the impact of NTRC and Trx f deficiency on starch metabolism by generating Nicotiana benthamiana plants that were silenced for each gene. Our results demonstrated that NTRC overexpression induced enhanced starch accumulation in tobacco leaves, as occurred with Trx f. However, only Trx f

SUBMITTER: Ancin M 

PROVIDER: S-EPMC6963466 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature