Quantification of Enterocytozoon hepatopenaei (EHP) in Penaeid Shrimps from Southeast Asia and Latin America Using TaqMan Probe-Based Quantitative PCR.

ABSTRACT: We developed a qPCR assay based on the β-tubulin gene sequence for the shrimp microsporidian parasite Enterocytozoon hepatopenaei (EHP). This assay reacted with the hepatopancreas (HP) of EHP-infected shrimps, and the highest copy numbers were found in HP and feces samples from Southeast Asian countries (10⁶-10⁸ copies mg^-1), while HP samples from Latin America, Artemia, and EHP-contaminated water showed lower amounts (10¹-10³ copies mg^-1 or mL^-1 of water). No false positive was found with the normal shrimp genome, live feeds, or other parasitic diseases. This tool will facilitate the management of EHP infection in shrimp farms.

SUBMITTER: Piamsomboon P 

PROVIDER: S-EPMC6963587 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature