A Marker-Free Bordetella bronchisepticaaroA/bscN Double Deleted Mutant Confers Protection Against Lethal Challenge.

ABSTRACT: Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to inactivated vaccines and/or sub-unit vaccines, however, their safety is not up to the mark. In present study, we constructed marker-free aroA/bscN double deleted B. bronchiseptica QH09 through two-step homologous recombination strategy. Our data showed that QH09 attenuated virulence to mice compared with the parent aroA deleted B. bronchiseptica QH0814. We also found that QH09 meets the vaccine safety standards, upon challenge in piglets, did not cause any visible clinical signs or lesions on o

SUBMITTER: Ai W 

PROVIDER: S-EPMC6963861 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature