Voltage-energized Calcium-sensitive ATP Production by Mitochondria.

ABSTRACT: Regulation of ATP production by mitochondria, critical to multicellular life, is poorly understood. Here we investigate the molecular controls of this process in heart and provide a framework for its Ca²⁺-dependent regulation. We find that the entry of Ca²⁺ into the matrix through the mitochondrial calcium uniporter (MCU) in heart has neither an apparent cytosolic Ca²⁺ threshold nor gating function and guides ATP production by its influence on the inner mitochondrial membrane (IMM) potential, ΔΨ_m. This regulation occurs by matrix Ca²⁺-dependent modulation of pyruvate and glutamate dehydrogenase activity and not through any effect of Ca²⁺ on ATP Synthase or on Electron Transport Chain Complexes II, III or IV. Examining the ΔΨ

SUBMITTER: Wescott AP 

PROVIDER: S-EPMC6964030 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature