Single-cell transcriptomics from human pancreatic islets: sample preparation matters.

ABSTRACT: Single-cell RNA sequencing (scRNA-seq) of human primary tissues is a rapidly emerging tool for investigating human health and disease at the molecular level. However, optimal processing of solid tissues presents a number of technical and logistical challenges, especially for tissues that are only available at autopsy, which includes pancreatic islets, a tissue that is highly relevant to diabetes. To assess the possible effects of different sample preparation protocols on fresh islet samples, we performed a detailed comparison of scRNA-seq data generated with islets isolated from a human donor but processed according to four treatment strategies, including fixation and cryopreservation. We found significant and reproducible differences in the proportion of cell types identified, and more mi

SUBMITTER: Bonnycastle LL 

PROVIDER: S-EPMC6964677 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature