Phospholipase D2 in prostate cancer: protein expression changes with Gleason score.

ABSTRACT:

Background

Phospholipases D1 and D2 (PLD1/2) are implicated in tumorigenesis through their generation of the signalling lipid phosphatidic acid and its downstream effects. Inhibition of PLD1 blocks prostate cell growth and colony formation. Here a role for PLD2 in prostate cancer (PCa), the major cancer of men in the western world, is examined.

Methods

PLD2 expression was analysed by immunohistochemistry and western blotting. The effects of PLD2 inhibition on PCa cell viability and cell motility were measured using MTS, colony forming and wound-healing assays.

Results

PLD2 protein is expressed about equally in luminal and basal prostate epithelial cells. In cells from different Gleason-scored PCa tissue PLD2 protein expression is generally higher than in non-tumorigen

SUBMITTER: Noble AR 

PROVIDER: S-EPMC6964697 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature