Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro.

ABSTRACT: In this study, we describe a new in vitro tick feeding system that facilitates the study of ticks and tick-borne pathogens. To optimize the system, we used Dermacentor andersoni and Anaplasma marginale as a tick-pathogen interaction model. Ticks were fed on bovine blood containing 10-fold dilutions of the pathogen to determine the effect of dose on tick infection rate. After feeding on infected blood, ticks were transferred to uninfected blood to stimulate bacterial replication within the tick vector. During stimulation feeding, blood samples were collected daily to determine if infected ticks secreted viable A. marginale. The results demonstrated similar attachment rates between the first and second tick feeding. Tick midgut and salivary glands were infected with A. marginale. However, sa

SUBMITTER: Vimonish R 

PROVIDER: S-EPMC6965182 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature