Leukocyte-derived extracellular DNA contributes to abnormal pressure elevation in the extracorporeal circulation circuit.

ABSTRACT: An abnormal elevation in pressure is a serious complication involving the extracorporeal circulation circuit. Clot formation might be associated with this complication, but the precise mechanism of an abnormal elevation in pressure has not been identified. We investigated sufficient conditions for in-circuit elevation in pressure using an ex vivo re-circulation circuit with porcine blood. Specifically, we investigated the effect of blood conditions, the type of anticoagulation, and pro-inflammatory stimulation on in-circuit pressure. We also examined the cause of an abnormal elevation of in-circuit pressure by specifically degrading DNA, RNA, or protein components of an obstructed filter and by using immunofluorescent techniques. Neither a change in temperature nor change in pH in the bloo

SUBMITTER: Yashima N 

PROVIDER: S-EPMC6965310 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature