Novel Hyperthermophilic Crenarchaeon Thermofilum adornatum sp. nov. Uses GH1, GH3, and Two Novel Glycosidases for Cellulose Hydrolysis.

ABSTRACT: A novel hyperthermophilic, anaerobic filamentous archaeon, Thermofilum adornatum strain 1910b^T, is capable of growing with cellulose as its sole carbon and energy source. This strain was isolated from a terrestrial hot spring in Kamchatka, Russia. The isolate 1910b^T grew optimally at a temperature of 80°C and a pH of 5.5-6.0, producing cell-bound inducible cellulases. During genome analysis, genes, encoding various glycosidases (GHs) involved in oligo- and polysaccharide hydrolysis and genes for the fermentation of sugars were identified. No homologs of currently known cellulase families were found among the GHs encoded by the 1910b^T genome, suggesting that novel proteins are involved. To figure this out, a proteomic analysis of cells grown on cellul

SUBMITTER: Zayulina KS 

PROVIDER: S-EPMC6965361 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature