Characterisation of nerve-mediated ATP release from bladder detrusor muscle and its pathological implications.

ABSTRACT:

Background and purpose

This study aims to characterise the molecular mechanisms that determine variability of atropine resistance of nerve-mediated contractions in human and guinea pig detrusor smooth muscle.

Experimental approach

Atropine resistance of nerve-mediated contractions and the role of P2X1 receptors, were assessed in isolated preparations from guinea pigs and also humans with or without overactive bladder syndrome, from which the mucosa was removed. Nerve-mediated ATP release was measured directly with amperometric ATP-sensitive electrodes. Ecto-ATPase activity of guinea pig and human detrusor samples was measured in vitro by measuring the concentration-dependent rate of ATP breakdown. The transcription of ecto-ATPase subtypes in human samples was measured by qPC

SUBMITTER: McCarthy CJ 

PROVIDER: S-EPMC6965683 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature