MiR-124 enhances cell radiosensitivity by targeting PDCD6 in nasopharyngeal carcinoma.

ABSTRACT: BACKGROUND:Radiation resistance poses a major clinical challenge in treatment of nasopharyngeal carcinoma (NPC). Studies have shown that the abnormal expression of microRNAs (miRNAs) is associated with radiosensitivity, however, the mechanisms have not been fully elucidated. The aim of this study, therefore, was to investigate whether ectopic expression of miR-124 is correlated with radiosensitivity in NPC. METHODS:In this study, the expression level of miR-124 was evaluated in NPC cell lines and patient specimens using quantitative reverse transcription-PCR (Real-time qPCR). Cell radiosensitivity was determined by colony formation assay. Target prediction algorithms and luciferase assay were used to confirm the target of miR-124. Tumor xenograft model was performed to understand the funct

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6966029 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature