Mediators of hypoxia in a rat model of sterile-induced acute liver injury.

ABSTRACT: BACKGROUND:The liver plays a key role in iron homeostasis during injury and hypoxia. METHODS:For induction of liver injury, thioacetamide (TAA) was administered intraperitoneally to male Sprague Dawley rats. Animals were sacrificed at 0, 1, 3, 6, 12, 24, 48, 72 and 96 h. Serum, liver, spleen and heart tissues were collected from control and TAA-treated rats. Tissue sections were prepared for immunohistochemical studies. Nuclear and cytoplasmic proteins were isolated for Western blot analysis. RESULTS:Hypoxia inducible factor (HIF)-1α and ED1 positive cells accumulated around the portal field and the interlobular space within 12 hours after TAA administration. Accordingly, Western blot analysis of liver tissue showed an early increase of HIF1α followed by a decrease at 48 h to 96 h. For Ery

SUBMITTER: Ahmad S 

PROVIDER: S-EPMC6966030 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature