Mechanistic Signatures of Human Papillomavirus Insertions in Anal Squamous Cell Carcinomas.

ABSTRACT: The role of human papillomavirus (HPV) in anal squamous cell carcinoma (ASCC) carcinogenesis has been clearly established, involving the expression of viral oncoproteins and optional viral DNA integration into the host genome. In this article, we describe the various mechanisms and sites of HPV DNA insertion and assess their prognostic and predictive value in a large series of patients with HPV-positive ASCC with long-term follow-up. We retrospectively analyzed 96 tumor samples from 93 HPV-positive ASCC patients using the Capture-HPV method followed by Next-Generation Sequencing, allowing determination of HPV genotype and identification of the mechanisms and sites of viral genome integration. We identified five different mechanistic signatures of HPV insertions. The distribution of HPV sig

SUBMITTER: Morel A 

PROVIDER: S-EPMC6966520 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature