Enteric infection induces Lark-mediated intron retention at the 5' end of Drosophila genes.

ABSTRACT:

Background

RNA splicing is a key post-transcriptional mechanism that generates protein diversity and contributes to the fine-tuning of gene expression, which may facilitate adaptation to environmental challenges. Here, we employ a systems approach to study alternative splicing changes upon enteric infection in females from classical Drosophila melanogaster strains as well as 38 inbred lines.

Results

We find that infection leads to extensive differences in isoform ratios, which results in a more diverse transcriptome with longer 5' untranslated regions (5'UTRs). We establish a role for genetic variation in mediating inter-individual splicing differences, with local splicing quantitative trait loci (local-sQTLs) being preferentially located at the 5' end of transcripts and dir

SUBMITTER: Bou Sleiman M 

PROVIDER: S-EPMC6966827 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature