A novel approach for T7 bacteriophage genome integration of exogenous DNA.

ABSTRACT:

Background

The comparatively small genome, well elucidated functional genomics and rapid life cycle confer T7 bacteriophage with great advantages for bio-application. Genetic manipulation of T7 genome plays a key role in T7 related applications. As one of the important aspects in T7 phage genetic modification, gene knock-in refers to two main approaches including direct genetic manipulation in vitro and recombineering. Neither of these available methods are efficient enough to support the development of innovative applications capitalizing on T7 bio-system and thus there is room for novel strategies that address this issue. Integration mediated by the ΦC31 integrase is one of the most robust site-specific recombination systems. ΦC31 integrases with enhanced activity and specificity

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6966851 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature