The complex microbiome from native semen to embryo culture environment in human in vitro fertilization procedure.

ABSTRACT: BACKGROUND:Only a few microbial studies have conducted in IVF (in vitro fertilization), showing the high-variety bacterial contamination of IVF culture media to cause damage to or even loss of cultured oocytes and embryos. We aimed to determine the prevalence and counts of bacteria in IVF samples, and to associate them with clinical outcome. METHODS:The studied samples from 50 infertile couples included: raw (n = 48), processed (n = 49) and incubated (n = 50) sperm samples, and IVF culture media (n = 50). The full microbiome was analyzed by 454 pyrosequencing and quantitative analysis by real-time quantitative PCR. Descriptive statistics, t-, Mann-Whitney tests and Spearman's correlation were used for comparison of studied groups. RESULTS:The study involved normozoospermic men. Normal vagi

SUBMITTER: Stsepetova J 

PROVIDER: S-EPMC6966866 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature