MiR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment.

ABSTRACT:

Background

Vascular calcification is often associated with chronic inflammation and is a risk factor for brain arterial stiffness. Our previous results showed that miR32-5p was positively correlated with vascular smooth muscle cells (VSMC) calcification, but it is unclear whether miR32-5p promoted VSMC calcification by regulating inflammatory factor production.

Results

In this study, bioinformatics analysis was used to select tumour necrosis factor α (TNFα) as a candidate inflammatory factor associated with calcification. Moreover, alizarin red staining and qRT-PCR analysis revealed that TNFα produced by BV2 cells was the key promoting factor of VSMC calcification. Interestingly, the expression of TNFα was significantly increased at the mRNA and protein levels after miR32-5p

SUBMITTER: Cao J 

PROVIDER: S-EPMC6967090 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature