Alteration of the gut microbiota associated with childhood obesity by 16S rRNA gene sequencing.

ABSTRACT:

Background

Obesity is a global epidemic in the industrialized and developing world, and many children suffer from obesity-related complications. Gut microbiota dysbiosis might have significant effect on the development of obesity. The microbiota continues to develop through childhood and thus childhood may be the prime time for microbiota interventions to realize health promotion or disease prevention. Therefore, it is crucial to understand the structure and function of pediatric gut microbiota.

Methods

According to the inclusion criteria and exclusion criteria, twenty-three normal weight and twenty-eight obese children were recruited from Nanjing, China. Genomic DNA was extracted from fecal samples. The V4 region of the bacterial 16S rDNA was amplified by PCR, and sequencin

SUBMITTER: Chen X 

PROVIDER: S-EPMC6968493 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature