Microglial depletion and repopulation in brain slice culture normalizes sensitized proinflammatory signaling.

ABSTRACT: BACKGROUND:Microglia are critical mediators of neuroimmune pathology across multiple neurologic disorders. Microglia can be persistently activated or "primed" by Toll-like receptor (TLR) activation, ethanol, stress, and other insults. Thus, strategies to prevent or reverse microglial priming may be beneficial for conditions that involve progressively increasing microglial activation. Microglial depletion with repopulation is emerging as a potential therapy to normalize chronic immune activation. Primary organotypic hippocampal slice culture (OHSC) allows for the study of neuroimmune activation as well as microglial depletion and repopulation without involvement of peripheral immune activation. OHSC undergoes functional maturation and retains cytoarchitecture similar to in vivo. METHODS:OHS

SUBMITTER: Coleman LG 

PROVIDER: S-EPMC6969463 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature