Switching sides-Reengineered primary charge separation in the bacterial photosynthetic reaction center.

ABSTRACT: We report 90% yield of electron transfer (ET) from the singlet excited state P* of the primary electron-donor P (a bacteriochlorophyll dimer) to the B-side bacteriopheophytin (H_B) in the bacterial photosynthetic reaction center (RC). Starting from a platform Rhodobacter sphaeroides RC bearing several amino acid changes, an Arg in place of the native Leu at L185-positioned over one face of H_B and only ∼4 Å from the 4 central nitrogens of the H_B macrocycle-is the key additional mutation providing 90% yield of P⁺H_B^- This all but matches the near-unity yield of A-side P⁺H_A^- charge separation in the native RC. The 90% yield of ET to H_B derives from (minimally) 3 P* populations wi

SUBMITTER: Laible PD 

PROVIDER: S-EPMC6969525 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature