Successive passaging of a plant-associated microbiome reveals robust habitat and host genotype-dependent selection.

ABSTRACT: There is increasing interest in the plant microbiome as it relates to both plant health and agricultural sustainability. One key unanswered question is whether we can select for a plant microbiome that is robust after colonization of target hosts. We used a successive passaging experiment to address this question by selecting upon the tomato phyllosphere microbiome. Beginning with a diverse microbial community generated from field-grown tomato plants, we inoculated replicate plants across 5 plant genotypes for 4 45-d passages, sequencing the microbial community at each passage. We observed consistent shifts in both the bacterial (16S amplicon sequencing) and fungal (internal transcribed spacer region amplicon sequencing) communities across replicate lines over time, as well as a general lo

SUBMITTER: Morella NM 

PROVIDER: S-EPMC6969547 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature