Allopregnanolone Modulates GABAAR-Dependent CaMKIIδ3 and BDNF to Protect SH-SY5Y Cells Against 6-OHDA-Induced Damage.

ABSTRACT: Allopregnanolone (APα), as a functional neurosteroid, exhibits the neuroprotective effect on neurodegenerative diseases such as Parkinson's disease (PD) through γ-aminobutyric acid A receptor (GABAAR), but it has not been completely understood about its molecular mechanisms. In order to investigate the neuroprotective effect of APα, as well as to clarify its possible molecular mechanisms, SH-SY5Y neuronal cell lines were incubated with 6-hydroxydopamine (6-OHDA), which has been widely used as an in vitro model for PD, along with APα alone or in combination with GABAAR antagonist (bicuculline, Bic), intracellular Ca²⁺ chelator (EGTA) and voltage-gated L-type Ca²⁺ channel blocker (Nifedipine). The viability, proliferation, and differentiation of SH-SY5Y cells, th

SUBMITTER: Wang T 

PROVIDER: S-EPMC6970471 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature