Ontology highlight
ABSTRACT:
SUBMITTER: Chen J
PROVIDER: S-EPMC6970531 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
eLife 20191216
TraR and its homolog DksA are bacterial proteins that regulate transcription initiation by binding directly to RNA polymerase (RNAP) rather than to promoter DNA. Effects of TraR mimic the combined effects of DksA and its cofactor ppGpp, but the structural basis for regulation by these factors remains unclear. Here, we use cryo-electron microscopy to determine structures of <i>Escherichia coli</i> RNAP, with or without TraR, and of an RNAP-promoter complex. TraR binding induced RNAP conformationa ...[more]