Triple-cell lineage tracing by a dual reporter on a single allele.

ABSTRACT: Genetic lineage tracing is widely used to study organ development and tissue regeneration. Multicolor reporters are a powerful platform for simultaneously tracking discrete cell populations. Here, combining Dre-rox and Cre-loxP systems, we generated a new dual-recombinase reporter system, called Rosa26 traffic light reporter (R26-TLR), to monitor red, green, and yellow fluorescence. Using this new reporter system with the three distinct fluorescent reporters combined on one allele, we found that the readouts of the two recombinases Cre and Dre simultaneously reflect Cre⁺Dre^-, Cre^-Dre⁺, and Cre⁺Dre⁺ cell lineages. As proof of principle, we show specific labeling in three distinct progenitor/stem cell populations, inclu

SUBMITTER: Liu K 

PROVIDER: S-EPMC6970925 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature