Hypoxia induced LUCAT1/PTBP1 axis modulates cancer cell viability and chemotherapy response.

ABSTRACT:

Background

Hypoxic tumors are refractory to DNA damage drugs. However, the underlying mechanism has yet to be elucidated. We aimed to identify lncRNAs that upregulated under hypoxia and their effects on colorectal cancer (CRC).

Methods

CRC cells were treated with 1% O₂ to identify lncRNAs that upregulated under hypoxia. We integrated these lncRNAs with RNA-seq of 4 paired CRC tissues and TCGA data to get candidate lncRNAs. Multiple in vitro and in vivo assays were used to explore the role of LUCAT1 in CRC.

Results

We identified a hypoxia-induced lncRNA LUCAT1 that facilitated the growth of CRC cells and contributed to drug resistance of CRC cells both in vitro and in vivo. Mechanically, LUCAT1 interacts with polypyrimidine tract binding protein 1 (PTBP1) in

SUBMITTER: Huan L 

PROVIDER: S-EPMC6971890 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature