Recovery of viable endocrine-specific cells and transcriptomes from human pancreatic islet-engrafted mice.

ABSTRACT: Human pancreatic islets engrafted into immunodeficient mice serve as an important model for in vivo human diabetes studies. Following engraftment, islet function can be monitored in vivo by measuring circulating glucose and human insulin; however, it will be important to recover viable cells for more complex graft analyses. Moreover, RNA analyses of dissected grafts have not distinguished which hormone-specific cell types contribute to gene expression. We developed a method for recovering live cells suitable for fluorescence-activated cell sorting from human islets engrafted in mice. Although yields of recovered islet cells were relatively low, the ratios of bulk-sorted β, α, and δ cells and their respective hormone-specific RNA-Seq transcriptomes are comparable pretransplant and posttrans

SUBMITTER: Redick SD 

PROVIDER: S-EPMC6972551 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature