Expression and functional analysis of the hydrogen peroxide biosensors HyPer and HyPer2 in C2C12 myoblasts/myotubes and single skeletal muscle fibres.

ABSTRACT: Hydrogen peroxide (H₂O₂) is generated in cells and plays an important role as a signalling molecule. It has been reported that H₂O₂ is involved in physiological and pathological processes in skeletal muscle. However, H₂O₂ detection in cells with traditional techniques produces frequent artefacts. Currently, the HyPer biosensor detects intracellular H₂O₂ specifically in real time using fluorescence microscopy. The aim of this study was to develop and optimize approaches used to express the HyPer biosensor in different models of skeletal muscle cells, such as the C2C12 myoblast/myotube cell line and mature skeletal muscle fibres isolated from C57BL/6J mice, and to measure intracellular H₂O

SUBMITTER: Fernandez-Puente E 

PROVIDER: S-EPMC6972731 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature