Cation permeability in CorA family of proteins.

ABSTRACT: CorA proteins belong to 2-TM-GxN family of membrane proteins, and play a major role in Mg²⁺ transport in prokaryotes and eukaryotic mitochondria. The selection of substrate is believed to occur via the signature motif GxN, however there is no consensus how strict this selection within the family. To answer this question, we employed fluorescence-based transport assays on three different family members, namely CorA from bacterium Thermotoga maritima, CorA from the archeon Methanocaldococcus jannaschii and ZntB from bacterium Escherichia coli, reconstituted into proteoliposomes. Our results show that all three proteins readily transport Mg²⁺, Co²⁺, Ni²⁺ and Zn²⁺, but not Al³⁺. Despite the similarity in cation specificity, ZntB

SUBMITTER: Stetsenko A 

PROVIDER: S-EPMC6972854 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature