YqfB protein from Escherichia coli: an atypical amidohydrolase active towards N⁴-acylcytosine derivatives.

ABSTRACT: Human activating signal cointegrator homology (ASCH) domain-containing proteins are widespread and diverse but, at present, the vast majority of those proteins have no function assigned to them. This study demonstrates that the 103-amino acid Escherichia coli protein YqfB, previously identified as hypothetical, is a unique ASCH domain-containing amidohydrolase responsible for the catabolism of N⁴-acetylcytidine (ac4C). YqfB has several interesting and unique features: i) it is the smallest monomeric amidohydrolase described to date, ii) it is active towards structurally different N⁴-acylated cytosines/cytidines, and iii) it has a high specificity for these substrates (k_cat/K_m up to 2.8 × 10⁶ M^-1 s^-1). Moreover, ou

SUBMITTER: Stanislauskiene R 

PROVIDER: S-EPMC6972931 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature