MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB.

ABSTRACT:

Background

Growing evidence shows that dysregulation of miRNAs plays a significant role in papillary thyroid cancer (PTC) tumorigenesis and development. The abnormal expression of miR-384 has been acknowledged in the proliferation or metastasis of some cancers. However, the function and the underlying mechanism of miR-384 in PTC progression remain largely unknown.

Methods

Real-time PCR was conducted to detect miR-384 expression in 58 cases of PTC and their adjacent noncancerous tissues. MTT, soft agar assay Transwell assay, and wound-healing assay were carried out to explore the biological function of miR-384 in PTC cell lines of BCPAP and K1. Bioinformatics analysis, dual-luciferase reporter assay, western blot, and functional complementation analysis were conducted to expl

SUBMITTER: Wang Y 

PROVIDER: S-EPMC6973191 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature