A secretion biosensor for monitoring Sec-dependent protein export in Corynebacterium glutamicum.

ABSTRACT:

Background

In recent years, the industrial workhorse Corynebacterium glutamicum has gained increasing interest as a host organism for the secretory production of heterologous proteins. Generally, the yield of a target protein in the culture supernatant depends on a multitude of interdependent biological and bioprocess parameters which have to be optimized. So far, the monitoring of such optimization processes depends on the availability of a direct assay for the respective target protein that can be handled also in high throughput approaches. Since simple assays, such as standard enzymatic activity assays, are not always at hand, the availability of a general protein secretion biosensor is highly desirable.

Results

High level secretion of proteins via the Sec protein export

SUBMITTER: Jurischka S 

PROVIDER: S-EPMC6975037 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature