Real-time monitoring of glutathione in living cells using genetically encoded FRET-based ratiometric nanosensor.

ABSTRACT: Reduced glutathione (GSH) level inside the cell is a critical determinant for cell viability. The level of GSH varies across the cells, tissues and environmental conditions. However, our current understanding of physiological and pathological GSH changes at high spatial and temporal resolution is limited due to non-availability of practicable GSH-detection methods. In order to measure GSH at real-time, a ratiometric genetically encoded nanosensor was developed using fluorescent proteins and fluorescence resonance energy transfer (FRET) approach. The construction of the sensor involved the introduction of GSH binding protein (YliB) as a sensory domain between cyan fluorescent protein (CFP; FRET donor) and yellow fluorescent protein (YFP; FRET acceptor). The developed sensor, named as FLIP-G

SUBMITTER: Ahmad M 

PROVIDER: S-EPMC6976633 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature