Functional properties of human platelets derived in vitro from CD34⁺ cells.

ABSTRACT: The in vitro production of blood platelets for transfusion purposes is an important goal in the context of a sustained demand for controlled products free of infectious, immune and inflammatory risks. The aim of this study was to characterize human platelets derived from CD34⁺ progenitors and to evaluate their hemostatic properties. These cultured platelets exhibited a typical discoid morphology despite an enlarged size and expressed normal levels of the major surface glycoproteins. They aggregated in response to ADP and a thrombin receptor agonist peptide (TRAP). After infusion into NSG mice, cultured and native platelets circulated with a similar 24 h half-life. Notably, the level of circulating cultured platelets remained constant during the first two hours following infusion

SUBMITTER: Do Sacramento V 

PROVIDER: S-EPMC6976668 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature