Glucocorticoid induces human beta cell dysfunction by involving riborepressor GAS5 LincRNA.

ABSTRACT:

Objective

A widely recognized metabolic side effect of glucocorticoid (GC) therapy is steroid-induced diabetes mellitus (DM). However, studies on the molecular basis of GC-induced pancreatic beta cell dysfunction in human beta cells are lacking. The significance of non-coding RNAs in various cellular processes is emerging. In this study, we aimed to show the direct negative impact of GC on beta cell function and elucidate the role of riborepressor GAS5 lincRNA in the GC signaling pathway in human pancreatic beta cells.

Methods

Patients undergoing two weeks of high-dose prednisolone therapy were monitored for C-peptide levels. Human pancreatic islets and the human beta cell line EndoC-βH1 were incubated in pharmacological concentrations of dexamethasone. The GAS5 level was mo

SUBMITTER: Esguerra JLS 

PROVIDER: S-EPMC6976904 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature