Long non-coding RNA TGLC15 advances hepatocellular carcinoma by stabilizing Sox4.

ABSTRACT:

Background

The hepatocellular carcinoma (HCC) belongs to a common malignancy especially in China. Recent data have clarified important roles of long non-coding RNAs (lncRNAs) in HCC. However, the role of a novel intergenic lncRNA termed TGLC15 is still elusive.

Methods

We screened for novel lncRNAs using lncRNA profiling. TGLC15 expression was quantified by qRT-PCR. In vitro experiments such as migration and viability assays were performed. In vivo implantation experiments were conducted to investigate tumorigenic functions of TGLC15. Combined RNA immunoprecipitation (RIP) and mass spectrometry (MS) were utilized to uncover Sox4 as TGLC15 binding protein.

Results

TGLC15 is significantly overexpressed in tumor tissues and HCC cell lines. Higher TGLC15 levels correlated

SUBMITTER: Chen Y 

PROVIDER: S-EPMC6977111 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature